Protein purification from E. coli extracts.
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The recombinant enzymes were purified from the cell free extracts of E. coli expressing the respective enzymes.

SEEK ID: https://demo.fairdomhub.org/sops/18?version=1

Filename: SOP-Protein purification.docx

Format: Word document

Size: 111 KB

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Not specified

Submitter

Jacky Snoep

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Views: 6079 Downloads: 424

Created: 30th Jan 2013 at 23:38

Last updated: 28th May 2015 at 08:50

Last used: 23rd May 2022 at 00:56

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Version 1 Created 30th Jan 2013 at 23:38 by Jacky Snoep

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People (1)
Programmes (1)
Projects (1)
Investigations (1)
Studies (1)
Assays (4)

Jacky Snoep

Projects: SysMO-LAB, PSYSMO, MOSES, SulfoSys, SysMO DB

Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch

SysMO

SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".

The goal pursued by SysMO is to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.

Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...

Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB

Web page: http://sysmo.net/

SulfoSys

Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation

Programme: SysMO

Public web page: http://sulfosys.com/

Organisms: Sulfolobus solfataricus

Central Carbon Metabolism of Sulfolobus solfataricus

SulfoSys

All creators

Jacky Snoep

An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.

Submitter: Jacky Snoep

Studies: Carbon loss at high T, Model gluconeogenesis, Pathway optimisation

Assays: Experimental assays of the temperature dependent degradation of GAP and ..., FBPA/ase, FBPAase model, GAPDH, GAPDH modeling, Gluconeogenic model, Model reconstituted system, Modelling pathway optimisation, Modelling temperature dependent degradation of GAP and DHAP, PGK, PGK and FBPAase titrations, PGK modeling, Reconstituted system reference state, TPI, TPI modeling

Snapshots: Snapshot 1

Scales: Not specified

Created: 29th Jan 2013 at 14:09, Last updated: 28th May 2015 at 08:50

Model gluconeogenesis

SulfoSys

All creators

Jacky Snoep

Mathematical model of a subset of reactions comprising the three most temperature sensitive intermediates of the gluconeogenic pathway in S. solfataricus

Submitter: Jacky Snoep

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Assays: Experimental assays of the temperature dependent degradation of GAP and ..., FBPA/ase, FBPAase model, GAPDH, GAPDH modeling, Gluconeogenic model, Modelling temperature dependent degradation of GAP and DHAP, PGK, PGK modeling, TPI, TPI modeling