Protein purification from E. coli extracts. Version 1
The recombinant enzymes were purified from the cell free extracts of E. coli expressing the respective enzymes.
SEEK ID: https://demo.fairdomhub.org/sops/18?version=1
Filename:
SOP-Protein purification.docx
Format: Word document
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Contributor and Creators
Views: 1371 Downloads: 168
Created: 30th Jan 2013 at 00:38
Last updated: 28th May 2015 at 10:50
Last used: 15th Jul 2018 at 00:17
AttributionsNone
ScalesRelated items
Projects: PSYSMO, MOSES, SysMO DB, SysMO-LAB, SulfoSys
Institutions: Manchester Centre for Integrative Systems Biology, University of Manchester, University of Stellenbosch
Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Snapshots: Snapshot 1
Scales: Not specified
Mathematical model of a subset of reactions comprising the three most temperature sensitive intermediates of the gluconeogenic pathway in S. solfataricus
Person responsible: Jacky Snoep
Snapshots: No snapshots
Scales: Not specified
Kinetic characterization of the FBPA/ase with respect to GAP en DHAP saturation.
Contributor: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Snapshots: No snapshots
Scales: Not specified
Contributor: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Snapshots: No snapshots
Scales: Not specified
Contributor: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Snapshots: No snapshots
Scales: Not specified
Kinetic characterisation of Phosphoglycerokinase
Contributor: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Snapshots: No snapshots
Scales: Not specified
