Assays

Find some moths

Sharing well-annotated research data helps the systems biology process.

This practical is about how to easily structure your experimental data such that it becomes well annotated, standard compliant, reproducible, and re-usable (also for others). While much big data travels from machine to machine without human intervention, we will focus on exchange of small data describing experiments which is (to our experience) mostly handled via MS Excel and similar tools. First we give an outline talk about
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Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.

Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.

Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.

The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.

Mathematical model for the analysis of carbon loss of BPG, GAP and DHAP in reconstituted system of S. solfataricus at 70C.