Assays

28 Assays visible to you, out of a total of 43

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Bupalus

SysMO DB

Find some moths

Contributor: Guest

Biological problem addressed: Modelling Analysis

Investigation: The moth investigation

Study: The moth study

Organisms:

Models: No Models

SOPs: No SOPs

Data files: Moth data

Scales: Organism

Created: 5th Jun 2015 at 11:29, Last updated: 13th Jan 2016 at 15:08

Gluconeogenic model

SulfoSys

Mathematical model for the analysis of carbon loss of BPG, GAP and DHAP in reconstituted system of S. solfataricus at 70C.

Contributor: Jacky Snoep

Biological problem addressed: Modelling Analysis

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

Models: Mathematical model for the combined four enzyme...

SOPs: No SOPs

Data files: Metabolite concentrations during reconstituted ...

Scales: Not specified

Created: 2nd Feb 2013 at 14:21, Last updated: 28th May 2015 at 10:50

Modelling pathway optimisation

SulfoSys

Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.

Contributor: Jacky Snoep

Biological problem addressed: Modelling Analysis

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Pathway optimisation

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

Models: Mathematical model for the combined four enzyme...

SOPs: No SOPs

Data files: FBPAase titration of reconstituted system, Model simulation and experimental data for PGK ..., PGK titration of reconstituted system, Pathway efficiency for 3PG to F6P conversion as...

Scales: Not specified

Created: 3rd Feb 2013 at 10:56, Last updated: 28th May 2015 at 10:50

PGK and FBPAase titrations

SulfoSys

Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.

Contributor: Jacky Snoep

Assay type: Extracellular metabolite concentration

Technology type: Progressive curve experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Pathway optimisation

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: No SOPs

Data files: FBPAase titration of reconstituted system, Model simulation and experimental data for PGK ..., PGK titration of reconstituted system

Scales: Not specified

Created: 3rd Feb 2013 at 11:18, Last updated: 28th May 2015 at 10:50

Experimental assays of the temperature dependent degradation of GAP and DHAP

SulfoSys

GAP and DHAP concentrations were measured over time at 70C.

Contributor: Jacky Snoep

Assay type: Experimental Assay

Technology type: Progressive curve experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms:

SOPs: No SOPs

Data files: T degradation DHAP, T degradation GAP

Scales: Not specified

Created: 29th Jan 2013 at 22:01, Last updated: 28th May 2015 at 10:50

FBPA/ase

SulfoSys

Kinetic characterization of the FBPA/ase with respect to GAP en DHAP saturation.

Contributor: Jacky Snoep

Assay type: Experimental Assay

Technology type: Initial rate experiment

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.

Data files: FBPA/ase kinetics DHAP, FBPA/ase kinetics GAP, Model simulation and experimental data for DHAP...

Scales: Not specified

Created: 29th Jan 2013 at 21:51, Last updated: 28th May 2015 at 10:50

FBPAase model

SulfoSys

Mathematical model for FBPAase kinetics, saturation of GAP and DHAP

Contributor: Jacky Snoep

Biological problem addressed: Modelling Analysis

Investigation: Central Carbon Metabolism of Sulfolobus solfata...

Study: Model gluconeogenesis

Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)

Models: FBPAase kinetic model

SOPs: No SOPs

Data files: FBPA/ase kinetics DHAP, FBPA/ase kinetics GAP, Model simulation and experimental data for DHAP...

Scales: Not specified

Created: 1st Feb 2013 at 22:47, Last updated: 28th May 2015 at 10:50

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