SOPs

5 SOPs visible to you, out of a total of 12

Cloning and heterologous expression of gluconeogenic enzymes in E. coli

Creator: Theresa Kouril

Contributor: Jacky Snoep

Preparation of cell free extracts of the recombinant E. coli strains expressing the respective S. solfataricus enzymes.

Creator: Theresa Kouril

Contributor: Jacky Snoep

The recombinant enzymes were purified from the cell free extracts of E. coli expressing the respective enzymes.

Creator: Theresa Kouril

Contributor: Jacky Snoep

A stationary-phase culture (10ml) was used to inoculate the fermenter vessel.

Taken from ArrayExpress:
http://www.ebi.ac.uk/microarray-as/ae/browse.html?keywords=E-MEXP-115

Creator: Katy Wolstencroft

Contributor: Katy Wolstencroft

The procedure describes the preparation of fluorescent DNA probes from human mRNA or total
RNA.

Creator: Olga Krebs

Contributor: Olga Krebs

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