P2
Not specified
Not specified
Not specified
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wild-type
wild-type
Views: 1174
Created: 28th Apr 2010 at 18:17
Last updated: 18th Jul 2012 at 14:26
Related items
Kinetic characterisation of Phosphoglycerokinase
Contributor: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Snapshots: No snapshots
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: Expression of S. solfataricus enzymes in E. col..., Preparation of cell free extract of recombinant..., Protein purification from E. coli extracts.
Data files: Model simulation and experimental data for ADP,..., PGK kinetics 3PG, PGK kinetics ADP, PGK kinetics ATP, PGK kinetics BPG
Scales: Not specified
Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.
Contributor: Jacky Snoep
Assay type: Extracellular Metabolite Concentration
Technology type: Progressive Curve Experiment
Snapshots: No snapshots
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Pathway optimisation
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: No SOPs
Data files: FBPAase titration of reconstituted system, Model simulation and experimental data for PGK ..., PGK titration of reconstituted system
Scales: Not specified
Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Pathway optimisation
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
Models: Mathematical model for the combined four enzyme...
SOPs: No SOPs
Data files: FBPAase titration of reconstituted system, Model simulation and experimental data for PGK ..., PGK titration of reconstituted system, Pathway efficiency for 3PG to F6P conversion as...
Scales: Not specified
Mathematical model for the analysis of carbon loss of BPG, GAP and DHAP in reconstituted system of S. solfataricus at 70C.
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
Models: Mathematical model for the combined four enzyme...
SOPs: No SOPs
Data files: Metabolite concentrations during reconstituted ...
Scales: Not specified
Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
Models: Mathematical model for the combined four enzyme...
SOPs: No SOPs
Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...
Scales: Not specified