The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
SEEK ID: https://demo.fairdomhub.org/assays/27
Experimental assay
Projects: SulfoSys
Investigation: Central Carbon Metabolism of Sulfolobus solfataricus
Study: Carbon loss at high T
Assay position:
Assay type: Experimental Assay Type
Technology type: Progressive Curve Experiment
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
Export PNG
Views: 5683
Created: 2nd Feb 2013 at 16:06
Last updated: 26th Oct 2017 at 09:57
This item has not yet been tagged.
Related items
- People (1)
- Programmes (1)
- Projects (1)
- Investigations (1)
- Studies (1)
- Strains (1)
- Data files (2)
- Publications (1)
SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".
The goal pursued by SysMO is to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.
Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...
Projects: BaCell-SysMO, COSMIC, SUMO, KOSMOBAC, SysMO-LAB, PSYSMO, SCaRAB, MOSES, TRANSLUCENT, STREAM, SulfoSys, SysMO DB
Web page: http://sysmo.net/
Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
Organisms: Sulfolobus solfataricus
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Submitter: Jacky Snoep
Studies: Carbon loss at high T, Model gluconeogenesis, Pathway optimisation
Assays: Experimental assays of the temperature dependent degradation of GAP and ..., FBPA/ase, FBPAase model, GAPDH, GAPDH modeling, Gluconeogenic model, Model reconstituted system, Modelling pathway optimisation, Modelling temperature dependent degradation of GAP and DHAP, PGK, PGK and FBPAase titrations, PGK modeling, Reconstituted system reference state, TPI, TPI modeling
Snapshots: Snapshot 1
Submitter: Jacky Snoep
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Assays: Model reconstituted system, Reconstituted system reference state
Snapshots: No snapshots
Submitter: Not available
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Sulfolobus solfataricus
Genotypes: wild-type
Phenotypes: wild-type
Comment: Not specified
Experimental data for the reconstituted system are plotted together with the model prediction
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Carbon loss at high T
Assays: Model reconstituted system, Reconstituted system reference state
The purified enzymes, PGK, GAPDH, TPI and FBPAase were incubated at 70 C en conversion of 3PG to F6P was followed
Abstract (Expand)
Authors: T. Kouril, D. Esser, J. Kort, H. V. Westerhoff, B. Siebers, J. L. Snoep
Date Published: 22nd Aug 2013
Publication Type: Not specified
PubMed ID: 23865479
Citation: FEBS J. 2013 Sep;280(18):4666-80. doi: 10.1111/febs.12438. Epub 2013 Aug 22.