Pathway optimisation

SysMO is a European transnational funding and research initiative on "Systems Biology of Microorganisms".

The goal pursued by SysMO is to record and describe the dynamic molecular processes going on in unicellular microorganisms in a comprehensive way and to present these processes in the form of computerized mathematical models.

Systems biology will raise biomedical and biotechnological research to a new quality level and contribute markedly to progress in understanding. Pooling European research ...

Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation

An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.

Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.

Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.

Combined plot fo the model simulations and the experimental data for the PGK and FBPAase titrations.

Model simulation showing the effect of varying PGK or FBPAase concentration on the efficiency for 3PG to F6P conversion.

Increasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.

Increasing the amount of FBPAase in the reconstituted system and measuring the 3PG and F6P fluxes.

The PGK, GAPDH, TPI and FBPAase were modelled together using the individual rate equations. Closed system.