Technology type 'Enzymatic Activity Measurements'

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8 Assays visible to you, out of a total of 8

Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer

GAP and DHAP concentrations were measured over time at 70C.

Contributor: Jacky Snoep

Assay type: Experimental Assay Type

Technology type: Progressive Curve Experiment

Snapshots: No snapshots

The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.

Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.

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