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Experimental data for the reconstituted system are plotted together with the model prediction
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Created: 2nd Feb 2013 at 21:55
Last updated: 28th May 2015 at 10:50
Last used: 17th Feb 2019 at 11:21

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Silicon cell model for the central carbohydrate metabolism of the archaeon Sulfolobus solfataricus under temperature variation
Programme: SysMO
Public web page: http://sulfosys.com/
Organisms: Sulfolobus solfataricus
An investigation in the CCM of S. solfataricus with a focus on the unique temperature adaptations and regulation; using a combined modelling and experimental approach.
Snapshots: Snapshot 1
Studies: Carbon loss at high T, Model gluconeogenesis, Pathway optimisation
Assays: Experimental assays of the temperature dependent degradation of GAP and ..., FBPA/ase, FBPAase model, GAPDH, GAPDH modeling, Gluconeogenic model, Model reconstituted system, Modelling pathway optimisation, Modelling temperature dependent degradation of GAP and DHAP, PGK, PGK and FBPAase titrations, PGK modeling, Reconstituted system reference state, TPI, TPI modeling
Scales: Not specified
Person responsible: Jacky Snoep
Snapshots: No snapshots
Scales: Not specified
Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
Models: Mathematical model for the combined four enzyme...
SOPs: No SOPs
Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...
Scales: Not specified
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
Contributor: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Progressive Curve Experiment
Snapshots: No snapshots
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Organisms: Sulfolobus solfataricus : P2 (wild-type / wild-type)
SOPs: No SOPs
Data files: Metabolite concentrations during reconstituted ..., Model simulation and Exp data for reconstituted...
Scales: Not specified
Abstract (Expand)
Authors: Jacky Snoep, Kouril T.,Esser D.,Kort J.,Westerhoff H. V.,Siebers B.
Date Published: 22nd Aug 2013
Journal: FEBS J
PubMed ID: 23865479
Citation: FEBS J. 2013 Sep;280(18):4666-80. doi: 10.1111/febs.12438. Epub 2013 Aug 22.
Scales: Not specified