Teusink

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This model is for a paper that examines whether the in vivo behavior of yeast glycolysis can be understood in terms of the in vitro kinetic properties of the constituent enzymes. In non-growing, anaerobic, compressed Saccharomyces cerevisiae the values of the kinetic parameters of most glycolytic enzymes were determined. For the other enzymes appropriate literature values were collected. By inserting these values into a kinetic model for glycolysis, fluxes and metabolites were calculated. Under the same conditions fluxes and metabolite levels were measured. In our first model, branch reactions were ignored. This model failed to reach the stable steady state that was observed in the experimental flux measurements.

Introduction of branches towards trehalose, glycogen, glycerol and succinate did allow such a steady state. The predictions of this branched model were compared with the empirical behavior. Half of the enzymes matched their predicted flux in vivo within a factor of 2. For the other enzymes it was calculated what deviation between in vivo and in vitro kinetic characteristics could explain the discrepancy between in vitro rate and in vivo flux.

SEEK DEMO ID: https://demo.sysmo-db.org/models/1?version=1

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  • Teusink.xml (XML document - 115 KB) Zoom Download

Organism: Saccharomyces cerevisiae

Model type: Not specified

Model format: SBML

Execution or visualisation environment: Not specified

Model image: No image specified

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Views: 1435   Downloads: 161

Created: 9th Jun 2009 at 16:46

Last updated: 21st Apr 2015 at 12:21

Last used: 29th Jun 2017 at 10:27

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help Scales
  • for scale Genomics passes gluc2 with unit of mg
  • for scale Aveoli passes width with unit of mm

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