Preparation of cell free extracts of the recombinant E. coli strains expressing the respective S. solfataricus enzymes.
Contributor: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model gluconeogenesis
Assays: FBPA/ase, GAPDH, PGK, TPI
The recombinant enzymes were purified from the cell free extracts of E. coli expressing the respective enzymes.