Assays

Glycolysis in Saccharomyces cerevisiae

Detailed glycolytic model in Lactococcus lactis

A kinetic model of the glycerol synthesis pathway has been constructed. Kinetic parameters were collected from published values. Maximal enzyme activities and intracellular effector concentrations were determined experimentally.

An assay to measure exttracellular glycerol using HPLC

Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer

Sharing well-annotated research data helps the systems biology process.

This practical is about how to easily structure your experimental data such that it becomes well annotated, standard compliant, reproducible, and re-usable (also for others). While much big data travels from machine to machine without human intervention, we will focus on exchange of small data describing experiments which is (to our experience) mostly handled via MS Excel and similar tools. First we give an outline talk about ...