Saturation of PGK with 3PG
Contributor: Jacky Snoep
Investigations: Central Carbon Metabolism of Sulfolobus solfata...
Studies: Model gluconeogenesis
Assays: PGK, PGK modeling
Kinetic characterisation of Phosphoglycerokinase. Measures enzyme reaction rate with increasing concentration of ATP
Increasing the amount of PGK in the incubation assay and measuring the 3PG and F6P fluxes.
Creator: Jacky Snoep
Studies: Pathway optimisation
Assays: Modelling pathway optimisation, PGK and FBPAase titrations
Model simulation showing the effect of varying PGK or FBPAase concentration on the efficiency for 3PG to F6P conversion.
Assays: Modelling pathway optimisation