Assays

3 Assays visible to you, out of a total of 3

SysMO DB

A kinetic model of the glycerol synthesis pathway has been constructed. Kinetic parameters were collected from published values. Maximal enzyme activities and intracellular effector concentrations were determined experimentally.

Submitter: Katy Wolstencroft

Biological problem addressed: Metabolism

Investigation: Glycerol Synthesis in Saccharomyces cerevisiae

Study: Glycerol Synthesis Pathway

Organisms: Saccharomyces cerevisiae

Models: Metabolic Control Analysis of Glycerol Synthesi...

SOPs: No SOPs

Data files: Cronwright Parameter Data

Snapshots: No snapshots

Created: 19th Aug 2011 at 09:23, Last updated: 26th Oct 2017 at 09:57

Extracellular Glycerol

SysMO DB

An assay to measure exttracellular glycerol using HPLC

Submitter: Katy Wolstencroft

Assay type: Metabolite Profiling

Technology type: HPLC

Investigation: Glycerol Synthesis in Saccharomyces cerevisiae

Study: Glycerol Synthesis Pathway

Organisms: Saccharomyces cerevisiae

SOPs: No SOPs

Data files: 1 hidden item

Snapshots: No snapshots

Created: 19th Aug 2011 at 10:02, Last updated: 26th Oct 2017 at 09:57

Intracellular Metabolites

SysMO DB

Intracellular metabolite concentrations were determined enzymatically by measuring the oxidation or reduction of NADH or NAD+, respectively, at 340 nm in a Beckman Coulter DU640 spectrophotometer

Submitter: Katy Wolstencroft

Assay type: Intracellular Metabolite Concentration

Technology type: Enzymatic Activity Measurements

Investigation: Glycerol Synthesis in Saccharomyces cerevisiae

Study: Glycerol Synthesis Pathway

Organisms: Saccharomyces cerevisiae

SOPs: No SOPs

Data files: Cronwright Parameter Data

Snapshots: No snapshots

Created: 19th Aug 2011 at 11:28, Last updated: 26th Oct 2017 at 09:57

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