Assays

What is an Assay?
15 Assays visible to you, out of a total of 16

GAP and DHAP concentrations were measured over time at 70C.

The temperature dependent degradation of DHAP and GAP is modelled as an exponential decay process using experimental data for the disintegration at 70C.

Mathematical model for FBPAase kinetics, saturation of GAP and DHAP

Mathematical model for PGK kinetics, saturation with ADP, ATP, 3PG and BPG.

Mathematical model for GAPDH kinetics, saturation of GAP, BPG, NADP, NADPH and Pi.

Mathematical model for TPI kinetics, saturation of GAP and DHAP.

Mathematical model for the analysis of carbon loss of BPG, GAP and DHAP in reconstituted system of S. solfataricus at 70C.

The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.

Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.

Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.

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