Assays
What is an Assay?Filters
Kinetic characterisation of Phosphoglycerokinase
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Kinetic characterization of the FBPA/ase with respect to GAP en DHAP saturation.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Initial Rate Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
GAP and DHAP concentrations were measured over time at 70C.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Progressive Curve Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
The temperature dependent degradation of DHAP and GAP is modelled as an exponential decay process using experimental data for the disintegration at 70C.
Submitter: Jacky Snoep
Biological problem addressed: Metabolism
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Mathematical model for FBPAase kinetics, saturation of GAP and DHAP
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Mathematical model for PGK kinetics, saturation with ADP, ATP, 3PG and BPG.
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Mathematical model for GAPDH kinetics, saturation of GAP, BPG, NADP, NADPH and Pi.
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Mathematical model for TPI kinetics, saturation of GAP and DHAP.
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
Mathematical model for the analysis of carbon loss of BPG, GAP and DHAP in reconstituted system of S. solfataricus at 70C.
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Model gluconeogenesis
The four purified enzymes were incubated in assay buffer and consumption of 3PG and production of F6P were measured in time, together with GAP and DHAP concentrations.
Submitter: Jacky Snoep
Assay type: Experimental Assay Type
Technology type: Progressive Curve Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Mathematical model for the reconstituted system with PGK, GAPDH, TPI and FBPAase.
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Carbon loss at high T
Varying the relative amounts of PGK and FBPAase and analyse the 3PG uptake and F6P production rates.
Submitter: Jacky Snoep
Biological problem addressed: Model Analysis Type
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Pathway optimisation
Experimental analysis of pathway fluxes with varying PGK and FBPAase concentrations in the incubation assay.
Submitter: Jacky Snoep
Assay type: Extracellular Metabolite Concentration
Technology type: Progressive Curve Experiment
Investigation: Central Carbon Metabolism of Sulfolobus solfata...
Study: Pathway optimisation